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antibody anti–activating transcription factor 6 (atf6; #65880)  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibody anti–activating transcription factor 6 (atf6; #65880)
    Antibody Anti–Activating Transcription Factor 6 (Atf6; #65880), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody anti–activating transcription factor 6 (atf6; #65880)/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    antibody anti–activating transcription factor 6 (atf6; #65880) - by Bioz Stars, 2026-03
    90/100 stars

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    Image Search Results


    Trichostatin A promotes epithelial-mesenchymal transition of esophageal squamous cell carcinoma cells by promoting endoplasmic reticulum stress. A: Western blot shows after trichostatin A (TSA) treatment, that endoplasmic reticulum (ER) stress marker glucose-regulated protein 78, C/EBP homologous protein and activating transcription factor 6 levels increased. These show that TSA can promote ER stress in esophageal cancer cells; B: Representative images of the migration of Eca109 and EC9706 cells treated with TSA combined with 4-phenylbutyric acid (4-PBA) from Transwell assays. The statistical results show that TSA enhanced cell migration reversed by 4-PBA in the Transwell migration assay; C: Western blot shows after TSA combined with 4-PBA, the epithelial-mesenchymal transition marker E-cadherin level increased, vimentin, β-catenin levels deceased. Cell counts are for the corresponding assays of at least five random microscope fields (100 magnification). n = 5, P vs control. a P < 0.05. b P < 0.01. Bar is 50 μm. TSA: Trichostatin A; 4-PBA: 4-phenylbutyric acid; GRP78: Glucose-regulated protein 78; CHOP: C/EBP homologous protein; ATF6: Activating transcription factor 6.

    Journal: World Journal of Gastroenterology

    Article Title: Trichostatin A augments cell migration and epithelial-mesenchymal transition in esophageal squamous cell carcinoma through BRD4 / c-Myc endoplasmic reticulum-stress pathway

    doi: 10.3748/wjg.v31.i11.103449

    Figure Lengend Snippet: Trichostatin A promotes epithelial-mesenchymal transition of esophageal squamous cell carcinoma cells by promoting endoplasmic reticulum stress. A: Western blot shows after trichostatin A (TSA) treatment, that endoplasmic reticulum (ER) stress marker glucose-regulated protein 78, C/EBP homologous protein and activating transcription factor 6 levels increased. These show that TSA can promote ER stress in esophageal cancer cells; B: Representative images of the migration of Eca109 and EC9706 cells treated with TSA combined with 4-phenylbutyric acid (4-PBA) from Transwell assays. The statistical results show that TSA enhanced cell migration reversed by 4-PBA in the Transwell migration assay; C: Western blot shows after TSA combined with 4-PBA, the epithelial-mesenchymal transition marker E-cadherin level increased, vimentin, β-catenin levels deceased. Cell counts are for the corresponding assays of at least five random microscope fields (100 magnification). n = 5, P vs control. a P < 0.05. b P < 0.01. Bar is 50 μm. TSA: Trichostatin A; 4-PBA: 4-phenylbutyric acid; GRP78: Glucose-regulated protein 78; CHOP: C/EBP homologous protein; ATF6: Activating transcription factor 6.

    Article Snippet: Membranes were initially blocked with 5% nonfat dry milk and subsequently incubated overnight at 4 °C with specific primary antibodies: acetylated histones H3 (acH3) (CST, 4353), H3 (CST, 4499), acetylated histones H4 (acH4) (Abcam, ab177790), H4 (Abcam, ab177840), N-cadherin (CST, 13116), E-cadherin (CST, 3195), vimentin (CST, 5741), β-catenin (CST, 8480), activating transcription factor 6 (ATF6) (Wanleibio, WL01153), C/EBP homologous protein (CHOP) (Wanleibio, WL00880), glucose-regulated protein (GRP) 78 (Wanleibio, WL03157), c-Myc (CST, 13987), and β-actin (CST, 4967) (all diluted 1:1000).

    Techniques: Western Blot, Marker, Migration, Transwell Migration Assay, Microscopy, Control